catastrophe! catastrophe!
- attrition occured with 3 EBs during media change
- iteratively revised stitch repair program isn’t working as intended (not to its most extent), and the original faulty ones from plate reader software directly is getting worse! quantitatively more frequent in failures!
- all col 12 EBs shrunk significantly! although b said it’s natural.
- my convo style perhaps sounded paranoid about attrition specifically, which makes me doubt the security of this collaboration on a social and mental level.
- i did an experiment with cellpose sam (domain specific variant of sam1) and it was significantly worse than sam3 mlx even when the latter is a general model. but the segmentation pipeline is well so this failed experiment is negligible in terms of its actual and psychological consequence.
but the worries of some of them are somewhat logically unfounded, and for the founded ones, especially attrition, bigger ebs tend to survive media change more due to their sheer size and weight. for the stitching, we shall see.
that shrinks down the amount of worries to one. segmentation pipeline is still well maintained, but stitching as i have described, is on a bit of a shaky ground.
to reduce every worry through logic and communication with domain expert (ie b) makes everything seem less catastrophic. catastrophe (whether real or purely psychological post-perception) seems to arise through the progressive stacking of small trivial problems. we are cpus afterall, and only worry when challenges arrive nonsequentially but in parallel, or simultaneously.
at this moment, everything is still going mostly in accordance. im in the storm and there’s no answer in sight yet (literally and figuratively). to document, attrition of 3 wells b2 4 5 happened on day 7.
We threw a party up in here, but God, it was bittersweet
I live hard 'cause I am scared that I won't mean anything
So now I'm praying to the ceiling, to the windows, to the walls
Against this sudden sinking feeling that there's nothing there at all
And still
We just persevere
— gang of youths